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Institut for Lægemiddeldesign og Farmakologi > Ansatte

Bente Vestergaard

Bente Vestergaard

Professor MSO

I am the group leader of the BioSAXS group, dept. Drug Design and Pharmacology, University of Copenhagen. have focused on structural biology throughout my carreer. I was trained in macromolecular X-ray crystallography and have experience with cryo electron microscopy, atomic force microscopy and fiber diffraction. Since 2004 I have specialized in small angle X-ray scattering on proteins in solution and have established the first Scandinavian bioSAXS group at the University of Copenhagen. In my group, we focus on structural characterisation of complex pharmaceutically relevant protein solutions. We analyze heterogeneous protein systems, and particularly specialize in characterisation of fibrillating proteins. We have developed novel methods for structural analysis of fibrillating systems, applying advanced decomposition and multidisciplinary characterisation.

 

 

Primære forskningsområder

BioSAXS

Structural Analysis of Protein Fibrillation

Structural Biology

Undervisnings- og vejledningsområder

National Teaching: course responsible for 'Advances in Medicinal Chemistry Research'. Lecturer and supervisor of practicals on 'Medicinal and Biostructural Chemistry'.

International Teaching: Co-organizer (together with my close colleague prof. Lise Arleth) of the Copenhagen BioSAS practial courses on biomacromolecular solution scattering. The courses aim at international young researchers (PhD, postdoc level) and is a collaboration with the group of EMBL group leader Dmitri Svergun and MAX-lab SAXS beamline (I911-4). I am returning teacher on the EMBL-Hamburg EMBO BioSAS courses, and (more recently) the ESRF Grenoble EMBO BioSAS course.

Udvalgte publikationer

  1. Udgivet

    A helical structural nucleus is the primary elongating unit of insulin amyloid fibrils

    Vestergaard, B., Jensen, M. G., Roessle, M., Kastrup, J. S. J., Van De Weert, M., Flink, J. M., Frøkjær, S., Gajhede, M. & Svergun, D. I. 2007 I : PLoS - Biology. 5, 5, s. 1089-1097

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  2. Udgivet

    Ionotropic glutamate-like receptor d2 binds D-serine and glycine.

    Naur, P., Hansen, K. B., Kristensen, A. S., Dravid, S. M., Pickering, D. S., Olsen, L., Vestergaard, B., Egebjerg, J., Gajhede, M., Traynelis, S. F. & Kastrup, J. S. J. 2007 I : Proceedings of the National Academy of Science of the United States of America. 104, 35, s. 14116-14121

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  3. Udgivet

    Small angle X-ray scattering study of calreticulin reveals conformational plasticity

    Toft, K. N., Larsen, N., Jørgensen, F. S., Højrup, P., Houen, G. & Vestergaard, B. 2008 I : Biochimica et Biophysica Acta. 1784, 9, s. 1265-1270

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  4. Udgivet

    High-throughput small angle X-ray scattering from proteins in solution using a microfluidic front-end

    Toft, K. N., Vestergaard, B., Nielsen, S. S., Snakenborg, D., Jeppesen, M. G., Jacobsen, J. K., Arleth, L. & Kutter, J. P. 2008 I : Analytical Chemistry. 80, 10, s. 3648-3654 7 s.

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  5. Udgivet

    Structures of the ligand-binding core of iGluR2 in complex with the agonists (R)- and (S)-2-amino-3-(4-hydroxy-1,2,5-thiadiazol-3-yl)propionic acid explain their unusual equipotency

    Beich-Frandsen, M., Pickering, D. S., Mirza, O., Johansen, T. N., Greenwood, J., Vestergaard, B., Schousboe, A., Gajhede, M., Liljefors, T. & Kastrup, J. S. 2008 I : Journal of Medicinal Chemistry. 51, 5, s. 1459-63

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  6. Udgivet

    Application of Bayesian analysis to indirect fourier transformation in small-angle scattering

    Vestergaard, B. & Hansen, S. L. 2006 I : Journal of Applied Crystallography. 39, s. 797-804 8 s.

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  7. Udgivet

    Small angle X-ray studies reveal that Aspergillus niger glucoamylase has a defined extended conformation and can form dimers in solution

    Jørgensen, A. D., Nøhr, J., Kastrup, J. S., Gajhede, M., Sigurskjold, B. W., Sauer, J., Svergun, D. I., Svensson, B. & Vestergaard, B. 2008 I : Journal of Biological Chemistry. 283, 21, s. 14772-14780

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  8. Udgivet

    A new small-angle X-ray scattering set-up on the crystallography beamline I711 at MAX-lab

    Knaapila, M., Svensson, C., Barauskas, J., Zackrisson, M., Nielsen, S. S., Toft, K. N., Vestergaard, B., Arleth, L., Olsson, U., Pedersen, J. S. & Cerenius, Y. 2009 I : Journal of Synchrotron Radiation. 16, 4, s. 498-504 7 s.

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  9. Udgivet

    BioXTAS RAW, a software program for high-throughput automated small-angle X-ray scattering data reduction and preliminary analysis

    Nielsen, S. S., Toft, K. N., Snakenborg, D., Jeppesen, M. G., Jacobsen, J. K., Vestergaard, B., Kutter, J. P. & Arleth, L. 2009 I : Journal of Applied Crystallography. 42, 5, s. 959-964 6 s.

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  10. Udgivet
  11. Udgivet

    Methods for structural characterization of prefibrillar intermediates and amyloid fibrils

    Langkilde, A. E. & Vestergaard, B. 2009 I : FEBS Letters. 583, 16, s. 2600-2609

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  12. Udgivet

    Extensive small-angle X-ray scattering studies of blood coagulation factor VIIa reveal interdomain flexibility

    Mosbæk, C. R., Nolan, D., Persson, E., Svergun, D. I., Bukrinsky, J. T. & Vestergaard, B. 2010 I : Biochemistry. 49, 45, s. 9739-9745

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  13. Udgivet

    Formation mechanism of insulin fibrils and structural aspects of the insulin fibrillation process

    Jensen, M. G., Frøkjær, S. & Vestergaard, B. 2009 I : Current Protein and Peptide Science. 10, 5, s. 509-528

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  14. Udgivet

    Self-association of long-acting insulin analogues studied by size exclusion chromatography coupled to multi-angle light scattering

    Jensen, M. H., Wahlund, P-O., Jacobsen, J. K., Vestergaard, B., van de Weert, M. & Havelund, S. 2011 I : Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. 879, 28, s. 2945-2951

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  15. Bacterial polypeptide release factor RF2 is structurally distinct from eukaryotic eRF1

    Vestergaard, B., Van, L. B., Andersen, G. R., Nyborg, J. & Kjeldgaard, M. 2001 I : Molecular Cell. 8, s. 1375-1382 7 s.

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  16. Recognition by tryptophanyl-tRNA synthetases of discriminator base on tRNATrp from three biological domains

    Guo, Q., Vestergaard, B., Costa, Degres, Wong, Grosjean, Zhu, Wong & Xue 2002 I : Journal of Biological Chemistry. 277, s. 14343-14349 6 s.

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  17. A cryo-electron microscopic study of ribosome-bound termination factor RF2

    Rawat, Zavialov, A., Sengupta, Valle, Grasucci, Linde, Vestergaard, B., Ehrenberg, M. & Frank, J. 2003 I : Nature. 421, s. 87-90 4 s.

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  18. Structure of the E. coli ribosomal termination complex with release factor 2

    Klaholz, B., Pape, T., Zavialov, A., Myasnikov, Orlova, Vestergaard, B., Ehrenberg, M. & van Heel, M. 2003 I : Nature. 421, s. 90-94 5 s.

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  19. Udgivet

    Crystal structure of the kainate receptor GluR5 ligand-binding core in complex with (S)-glutamate

    Naur, P., Vestergaard, B., Skov, L., Egebjerg, J., Gajhede, M. & Kastrup, J. S. J. 2005 I : FEBS Letters. 579, s. 1154-60

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  20. Udgivet

    Diverse bacterial genomes encode an operon of two genes, one of which is a unusual class-I release factor that potentially recognizes atypical mRNA signals other than normal stop codons

    Baranov, P., Vestergaard, B., Hamelryck, T. W., Gesteland, Nyborg, J. & Atking, J. 2006 I : Biology Direct. 13, s. 1-28 28 s.

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  21. Udgivet

    A high-affinity, dimeric inhibitor of PSD-95 bivalently interacts with PDZ1-2 and protects against ischemic brain damage

    Bach, A., Clausen, B. H., Møller, M., Vestergaard, B., Chi, C. N., Round, A., Sørensen, P. L., Nissen, K. B., Kastrup, J. S., Gajhede, M., Jemth, P., Kristensen, A. S., Lundström, P., Lambertsen, K. L. & Strømgaard, K. . C. 28 feb. 2012 I : National Academy of Sciences. Proceedings. 109, 9, s. 3317-3322

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  22. Udgivet

    Structural characterization of prefibrillar intermediates and amyloid fibrils by small-angle X-ray scattering

    Langkilde, A. E. & Vestergaard, B. 2012 Amyloid proteins: methods and protocols. Sigurdsson, E. M., Calero, M. & Gasset, M. (red.). 2 udg. New York: Springer Science+Business Media B.V., Vol. 849, Kap. 10, s. 137-155 18 s. (Methods in Molecular Biology, Vol. 849).

    Publikation: ForskningBidrag til bog/antologi

  23. Udgivet

    Small angle X-ray scattering-based elucidation of the self-association mechanism of human insulin analogue lys(B29)(N(e)¿-carboxyheptadecanoyl) des(B30)

    Jensen, M. H., Wahlund, P-O., Toft, K. N., Jacobsen, J. K., Steensgaard, D. B., van de Weert, M., Havelund, S. & Vestergaard, B. 15 jan. 2013 I : Biochemistry. 52, 2, s. 282-94 13 s.

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  24. Udgivet

    Large-scale polymorphism and auto-catalytic effect in insulin fibrillogenesis

    Foderà, V., van de Weert, M. & Vestergaard, B. 2010 I : Soft Matter. 6, 18, s. 4413-4419

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  25. Udgivet

    The SAXS Solution Structure of RF1 Differs from Its Crystal Structure and Is Similar to Its Ribosome Bound Cryo-EM Structure

    Vestergaard, B., Sanyal, S., Roessle, M., Mora, L., Buckingham, R., Kastrup, J. S. J., Gajhede, M., Svergun, D. I. & Ehrenberg, M. 2005 I : Molecular Cell. 20, s. 929-938

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  26. Udgivet

    Unlocked Concanavalin A Forms Amyloid-like Fibrils from Coagulation of Long-lived “Crinkled” Intermediates

    Vetri, V., Leone, M., Morozova-Roche, L. A., Vestergaard, B. & Foderà, V. 2013 I : PLoS ONE. 8, 7, 11 s., e68912

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  27. Udgivet

    Structural Investigations of on-pathway Oligomers of α-Synuclein

    Pedersen, M. N., Horvath, I., Weise, C. F., Foderà, V., Wittung-Stafshede, P., Wolf-Watz, M., Toft, K. N. & Vestergaard, B. 31 maj 2012

    Publikation: ForskningPoster

  28. Udgivet

    High concentration formulation studies of an IgG2 antibody using small angle X-ray scattering

    Mosbæk, C. R., Konarev, P., Svergun, D. I., Rischel, C. & Vestergaard, B. 2012 I : Pharmaceutical Research. 29, 8, s. 2225-2235

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  29. Udgivet

    Structural model and trans-interaction of the entire ectodomain of the olfactory cell adhesion molecule

    Kulahin, N., Kristensen, O., Rasmussen, K. K., Olsen, L., Rydberg, P., Vestergaard, B., Kastrup, J. S., Berezin, V., Bock, E., Walmod, P. S. & Gajhede, M. 9 feb. 2011 I : Structure. 19, 2, s. 203-211

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  30. Udgivet

    Automated microfluidic sample-preparation platform for high-throughput structural investigation of proteins by small-angle X-ray scattering

    Lafleur, J., Snakenborg, D., Nielsen, S. S., Møller, M., Toft, K. N., Menzel, A., Jacobsen, J. K., Vestergaard, B., Arleth, L. & Kutter, J. P. 2011 I : Journal of Applied Crystallography. 44, s. 1090-1099 10 s.

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  31. Udgivet

    Tyr702 is an important determinant of agonist binding and domain closure of the ligand-binding core of GluR2

    Frandsen, A., Pickering, D. S., Vestergaard, B., Kasper, C., Nielsen, B. B., Greenwood, J. R., Campiani, G., Fattorusso, C., Gajhede, M., Schousboe, A. & Kastrup, J. S. 2005 I : Molecular Pharmacology. 67, 3, s. 703-13 10 s.

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  32. Udgivet

    Low-resolution structure of a vesicle disrupting a-synuclein oligomer that accumulates during fibrillation

    Giehm, L., Svergun, D. I., Otzen, D. E. & Vestergaard, B. 2011 I : National Academy of Sciences. Proceedings. 108, 8, s. 3246-3251

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  33. Udgivet

    Time-resolved SAXS measurements facilitated by online HPLC buffer exchange

    Jensen, M. H., Toft, K. N., David, G., Havelund, S., Pérez, J. & Vestergaard, B. 2010 I : Journal of Synchrotron Radiation. 17, 6, s. 769-773

    Publikation: Forskning - fagfællebedømtTidsskriftartikel

  34. Udgivet

    Gennembrud i forståelsen af insulinfibrillering

    Jensen, M. G., Vestergaard, B., Frøkjær, S., Gajhede, M., Flink, J. M., Van De Weert, M., Kastrup, J. S. J. & Svergun, D. I. 2007 I : Lægemiddelforskning. s. 10-11

    Publikation: FormidlingTidsskriftartikel

  35. Udgivet

    Kimen til de farlige fibriller

    Jensen, M. G. & Vestergaard, B. 2007 I : Aktuel Naturvidenskab. 6, s. 12-15

    Publikation: FormidlingTidsskriftartikel

ID: 1305569