Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum

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Standard

Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum. / Domljanovic, Ivana; Rexen Ulven, Elisabeth; Ulven, Trond; Thomsen, Rasmus P.; Okholm, Anders H.; Kjems, Jørgen; Voss, Anne; Taskova, Maria; Astakhova, Kira.

I: ACS Omega, Bind 3, Nr. 7, 31.07.2018, s. 7580-7586.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Domljanovic, I, Rexen Ulven, E, Ulven, T, Thomsen, RP, Okholm, AH, Kjems, J, Voss, A, Taskova, M & Astakhova, K 2018, 'Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum', ACS Omega, bind 3, nr. 7, s. 7580-7586. https://doi.org/10.1021/acsomega.8b00424

APA

Domljanovic, I., Rexen Ulven, E., Ulven, T., Thomsen, R. P., Okholm, A. H., Kjems, J., Voss, A., Taskova, M., & Astakhova, K. (2018). Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum. ACS Omega, 3(7), 7580-7586. https://doi.org/10.1021/acsomega.8b00424

Vancouver

Domljanovic I, Rexen Ulven E, Ulven T, Thomsen RP, Okholm AH, Kjems J o.a. Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum. ACS Omega. 2018 jul. 31;3(7):7580-7586. https://doi.org/10.1021/acsomega.8b00424

Author

Domljanovic, Ivana ; Rexen Ulven, Elisabeth ; Ulven, Trond ; Thomsen, Rasmus P. ; Okholm, Anders H. ; Kjems, Jørgen ; Voss, Anne ; Taskova, Maria ; Astakhova, Kira. / Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum. I: ACS Omega. 2018 ; Bind 3, Nr. 7. s. 7580-7586.

Bibtex

@article{f007f0341f59482c92a313a5c9fc421e,
title = "Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum",
abstract = "Antigen recognition by antibodies plays an important role in human biology and in the development of diseases. This interaction provides a basis for multiple diagnostic assays and is a guide for treatments. We have developed dihydropyridine-based fluorophores that form stable complexes with double-stranded DNA and upon recognition of the antibodies to DNA (anti-DNA) provide an optical response. The fluorophores described herein have advantageous optical properties compared to those of the currently available dyes making them valuable for research and clinical diagnostics. By studying a series of novel fluorophores, crucial parameters for the design were established, providing the required sensitivity and specificity in the detection of antibodies. Using these DNA-fluorophore complexes in a direct immunofluorescence assay, antibodies to DNA are specifically detected in 80 patients diagnosed with an autoimmune disease, systemic lupus erythematosus. Positivity indicated by emission change of α-(4′-O-methoxyphenyl)-2-furyl dihydropyridine strongly correlates with other disease biomarkers and autoimmune arthritis.",
author = "Ivana Domljanovic and {Rexen Ulven}, Elisabeth and Trond Ulven and Thomsen, {Rasmus P.} and Okholm, {Anders H.} and J{\o}rgen Kjems and Anne Voss and Maria Taskova and Kira Astakhova",
year = "2018",
month = jul,
day = "31",
doi = "10.1021/acsomega.8b00424",
language = "English",
volume = "3",
pages = "7580--7586",
journal = "ACS Omega",
issn = "2470-1343",
publisher = "ACS Publications",
number = "7",

}

RIS

TY - JOUR

T1 - Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum

AU - Domljanovic, Ivana

AU - Rexen Ulven, Elisabeth

AU - Ulven, Trond

AU - Thomsen, Rasmus P.

AU - Okholm, Anders H.

AU - Kjems, Jørgen

AU - Voss, Anne

AU - Taskova, Maria

AU - Astakhova, Kira

PY - 2018/7/31

Y1 - 2018/7/31

N2 - Antigen recognition by antibodies plays an important role in human biology and in the development of diseases. This interaction provides a basis for multiple diagnostic assays and is a guide for treatments. We have developed dihydropyridine-based fluorophores that form stable complexes with double-stranded DNA and upon recognition of the antibodies to DNA (anti-DNA) provide an optical response. The fluorophores described herein have advantageous optical properties compared to those of the currently available dyes making them valuable for research and clinical diagnostics. By studying a series of novel fluorophores, crucial parameters for the design were established, providing the required sensitivity and specificity in the detection of antibodies. Using these DNA-fluorophore complexes in a direct immunofluorescence assay, antibodies to DNA are specifically detected in 80 patients diagnosed with an autoimmune disease, systemic lupus erythematosus. Positivity indicated by emission change of α-(4′-O-methoxyphenyl)-2-furyl dihydropyridine strongly correlates with other disease biomarkers and autoimmune arthritis.

AB - Antigen recognition by antibodies plays an important role in human biology and in the development of diseases. This interaction provides a basis for multiple diagnostic assays and is a guide for treatments. We have developed dihydropyridine-based fluorophores that form stable complexes with double-stranded DNA and upon recognition of the antibodies to DNA (anti-DNA) provide an optical response. The fluorophores described herein have advantageous optical properties compared to those of the currently available dyes making them valuable for research and clinical diagnostics. By studying a series of novel fluorophores, crucial parameters for the design were established, providing the required sensitivity and specificity in the detection of antibodies. Using these DNA-fluorophore complexes in a direct immunofluorescence assay, antibodies to DNA are specifically detected in 80 patients diagnosed with an autoimmune disease, systemic lupus erythematosus. Positivity indicated by emission change of α-(4′-O-methoxyphenyl)-2-furyl dihydropyridine strongly correlates with other disease biomarkers and autoimmune arthritis.

U2 - 10.1021/acsomega.8b00424

DO - 10.1021/acsomega.8b00424

M3 - Journal article

AN - SCOPUS:85049784084

VL - 3

SP - 7580

EP - 7586

JO - ACS Omega

JF - ACS Omega

SN - 2470-1343

IS - 7

ER -

ID: 209296331