Metabolism of prostaglandin E and of glutathione conjugate of prostaglandin A (GSH-prostaglandin A) by prostaglandin 9-ketoreductase from rabbit kidney
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Rabbit kidney prostaglandin 9-ketoreductase was found to metabolize the glutathione conjugate of prostaglandin A (GSH-prostaglandin A). Apparent K (GSH-prostaglandin A) 13 µM and apparent K (prostaglandin E) 200 µM. The cytosolic preparation was subjected to gelfiltration and isoelectric focusing, which revealed that metabolism of prostaglandin E and GSH-prostaglandin A occurs by means of the same fractions. Furthermore, prostaglandin E and GSH-prostaglandin A are competitive inhibitors of the enzyme, when GSH-prostaglandin A and prostaglandin E are tested as substrates, respectively. It is concluded, that GSH-prostaglandin A is a much better substrate for prostaglandin 9-ketoreductase from rabbit kidney than is prostaglandin E.
Originalsprog | Engelsk |
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Tidsskrift | B B A - Molecular and Cell Biology of Lipids |
Vol/bind | 574 |
Udgave nummer | 1 |
Sider (fra-til) | 33-38 |
Antal sider | 6 |
ISSN | 1388-1981 |
Status | Udgivet - 27 jul. 1979 |
ID: 45562933