Dual High-Resolution α-Glucosidase and Radical Scavenging Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of Minor and Major Constituents Directly from the Crude Extract of Pueraria lobata

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Dual High-Resolution α-Glucosidase and Radical Scavenging Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of Minor and Major Constituents Directly from the Crude Extract of Pueraria lobata. / Liu, Bingrui; Kongstad, Kenneth Thermann; Qinglei, Sun; Nyberg, Nils; Jäger, Anna; Stærk, Dan.

I: Journal of Natural Products, Bind 78, Nr. 2, 13.02.2015, s. 294–300.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Liu, B, Kongstad, KT, Qinglei, S, Nyberg, N, Jäger, A & Stærk, D 2015, 'Dual High-Resolution α-Glucosidase and Radical Scavenging Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of Minor and Major Constituents Directly from the Crude Extract of Pueraria lobata', Journal of Natural Products, bind 78, nr. 2, s. 294–300. https://doi.org/10.1021/np5009416

APA

Liu, B., Kongstad, K. T., Qinglei, S., Nyberg, N., Jäger, A., & Stærk, D. (2015). Dual High-Resolution α-Glucosidase and Radical Scavenging Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of Minor and Major Constituents Directly from the Crude Extract of Pueraria lobata. Journal of Natural Products, 78(2), 294–300. https://doi.org/10.1021/np5009416

Vancouver

Liu B, Kongstad KT, Qinglei S, Nyberg N, Jäger A, Stærk D. Dual High-Resolution α-Glucosidase and Radical Scavenging Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of Minor and Major Constituents Directly from the Crude Extract of Pueraria lobata. Journal of Natural Products. 2015 feb. 13;78(2):294–300. https://doi.org/10.1021/np5009416

Author

Liu, Bingrui ; Kongstad, Kenneth Thermann ; Qinglei, Sun ; Nyberg, Nils ; Jäger, Anna ; Stærk, Dan. / Dual High-Resolution α-Glucosidase and Radical Scavenging Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of Minor and Major Constituents Directly from the Crude Extract of Pueraria lobata. I: Journal of Natural Products. 2015 ; Bind 78, Nr. 2. s. 294–300.

Bibtex

@article{8496717ca487441a99c0124e1e1e984e,
title = "Dual High-Resolution α-Glucosidase and Radical Scavenging Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of Minor and Major Constituents Directly from the Crude Extract of Pueraria lobata",
abstract = "The crude methanol extract of Pueraria lobata was investigated by dual high-resolution α-glucosidase inhibition and radical scavenging profiling combined with hyphenated HPLC-HRMS-SPE-NMR. Direct analysis of the crude extract without preceding purification was facilitated by combining chromatograms from two analytical-scale HPLC separations of 120 and 600 μg on-column, respectively. High-resolution α-glucosidase and radical scavenging profiles were obtained after microfractionation of the eluate in 96-well microplates. This allowed full bioactivity profiling of individual peaks in the HPLC chromatogram of the crude methanol extract. Subsequent HPLC-HRMS-SPE-NMR analysis allowed identification of 21 known compounds in addition to two new compounds, i.e., 3′-methoxydaidzein 8-C-[α-d-apiofuranosyl-(1→6)]-β-d-glucopyranoside and 6″-O-malonyl-3′-methoxydaidzin, as well as an unstable compound tentatively identified as 3′-de-O-methylpuerariafuran.",
author = "Bingrui Liu and Kongstad, {Kenneth Thermann} and Sun Qinglei and Nils Nyberg and Anna J{\"a}ger and Dan St{\ae}rk",
year = "2015",
month = feb,
day = "13",
doi = "10.1021/np5009416",
language = "English",
volume = "78",
pages = "294–300",
journal = "Journal of Natural Products",
issn = "0163-3864",
publisher = "American Chemical Society",
number = "2",

}

RIS

TY - JOUR

T1 - Dual High-Resolution α-Glucosidase and Radical Scavenging Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of Minor and Major Constituents Directly from the Crude Extract of Pueraria lobata

AU - Liu, Bingrui

AU - Kongstad, Kenneth Thermann

AU - Qinglei, Sun

AU - Nyberg, Nils

AU - Jäger, Anna

AU - Stærk, Dan

PY - 2015/2/13

Y1 - 2015/2/13

N2 - The crude methanol extract of Pueraria lobata was investigated by dual high-resolution α-glucosidase inhibition and radical scavenging profiling combined with hyphenated HPLC-HRMS-SPE-NMR. Direct analysis of the crude extract without preceding purification was facilitated by combining chromatograms from two analytical-scale HPLC separations of 120 and 600 μg on-column, respectively. High-resolution α-glucosidase and radical scavenging profiles were obtained after microfractionation of the eluate in 96-well microplates. This allowed full bioactivity profiling of individual peaks in the HPLC chromatogram of the crude methanol extract. Subsequent HPLC-HRMS-SPE-NMR analysis allowed identification of 21 known compounds in addition to two new compounds, i.e., 3′-methoxydaidzein 8-C-[α-d-apiofuranosyl-(1→6)]-β-d-glucopyranoside and 6″-O-malonyl-3′-methoxydaidzin, as well as an unstable compound tentatively identified as 3′-de-O-methylpuerariafuran.

AB - The crude methanol extract of Pueraria lobata was investigated by dual high-resolution α-glucosidase inhibition and radical scavenging profiling combined with hyphenated HPLC-HRMS-SPE-NMR. Direct analysis of the crude extract without preceding purification was facilitated by combining chromatograms from two analytical-scale HPLC separations of 120 and 600 μg on-column, respectively. High-resolution α-glucosidase and radical scavenging profiles were obtained after microfractionation of the eluate in 96-well microplates. This allowed full bioactivity profiling of individual peaks in the HPLC chromatogram of the crude methanol extract. Subsequent HPLC-HRMS-SPE-NMR analysis allowed identification of 21 known compounds in addition to two new compounds, i.e., 3′-methoxydaidzein 8-C-[α-d-apiofuranosyl-(1→6)]-β-d-glucopyranoside and 6″-O-malonyl-3′-methoxydaidzin, as well as an unstable compound tentatively identified as 3′-de-O-methylpuerariafuran.

U2 - 10.1021/np5009416

DO - 10.1021/np5009416

M3 - Journal article

C2 - 25679337

VL - 78

SP - 294

EP - 300

JO - Journal of Natural Products

JF - Journal of Natural Products

SN - 0163-3864

IS - 2

ER -

ID: 130380572