Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks

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Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks. / Ignea, Codruta; Raadam, Morten Hessellund; Koutsaviti, Aikaterini; Zhao, Yong; Duan, Yaotao; Harizani, Maria; Miettinen, Karel; Georgantea, Panagiota; Rosenfeldt, Mads; Viejo-Ledesma, Sara E.; Petersen, Mikael Agerlin; Bredie, Wender; Stærk, Dan; Roussis, Vassilios; Ioannou, Efstathia; Kampranis, Sotirios.

I: Nature Communications, Bind 13, 5188, 2022.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Ignea, C, Raadam, MH, Koutsaviti, A, Zhao, Y, Duan, Y, Harizani, M, Miettinen, K, Georgantea, P, Rosenfeldt, M, Viejo-Ledesma, SE, Petersen, MA, Bredie, W, Stærk, D, Roussis, V, Ioannou, E & Kampranis, S 2022, 'Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks', Nature Communications, bind 13, 5188. https://doi.org/10.1038/s41467-022-32921-w

APA

Ignea, C., Raadam, M. H., Koutsaviti, A., Zhao, Y., Duan, Y., Harizani, M., Miettinen, K., Georgantea, P., Rosenfeldt, M., Viejo-Ledesma, S. E., Petersen, M. A., Bredie, W., Stærk, D., Roussis, V., Ioannou, E., & Kampranis, S. (2022). Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks. Nature Communications, 13, [5188]. https://doi.org/10.1038/s41467-022-32921-w

Vancouver

Ignea C, Raadam MH, Koutsaviti A, Zhao Y, Duan Y, Harizani M o.a. Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks. Nature Communications. 2022;13. 5188. https://doi.org/10.1038/s41467-022-32921-w

Author

Ignea, Codruta ; Raadam, Morten Hessellund ; Koutsaviti, Aikaterini ; Zhao, Yong ; Duan, Yaotao ; Harizani, Maria ; Miettinen, Karel ; Georgantea, Panagiota ; Rosenfeldt, Mads ; Viejo-Ledesma, Sara E. ; Petersen, Mikael Agerlin ; Bredie, Wender ; Stærk, Dan ; Roussis, Vassilios ; Ioannou, Efstathia ; Kampranis, Sotirios. / Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks. I: Nature Communications. 2022 ; Bind 13.

Bibtex

@article{b39f56a64a764a48a03f5511a69e1b49,
title = "Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks",
abstract = "Humankind relies on specialized metabolites for medicines, flavors, fragrances, and numerous other valuable biomaterials. However, the chemical space occupied by specialized metabolites, and, thus, their application potential, is limited because their biosynthesis is based on only a handful of building blocks. Engineering organisms to synthesize alternative building blocks will bypass this limitation and enable the sustainable production of molecules with non-canonical chemical structures, expanding the possible applications. Herein, we focus on isoprenoids and combine synthetic biology with protein engineering to construct yeast cells that synthesize 10 non-canonical isoprenoid building blocks with 16 carbon atoms. We identify suitable terpene synthases to convert these building blocks into C16 scaffolds and a cytochrome P450 to decorate the terpene scaffolds and produce different oxygenated compounds. Thus, we reconstruct the modular structure of terpene biosynthesis on 16-carbon backbones, synthesizing 28 different non-canonical terpenes, some of which have interesting odorant properties.",
author = "Codruta Ignea and Raadam, {Morten Hessellund} and Aikaterini Koutsaviti and Yong Zhao and Yaotao Duan and Maria Harizani and Karel Miettinen and Panagiota Georgantea and Mads Rosenfeldt and Viejo-Ledesma, {Sara E.} and Petersen, {Mikael Agerlin} and Wender Bredie and Dan St{\ae}rk and Vassilios Roussis and Efstathia Ioannou and Sotirios Kampranis",
year = "2022",
doi = "10.1038/s41467-022-32921-w",
language = "English",
volume = "13",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks

AU - Ignea, Codruta

AU - Raadam, Morten Hessellund

AU - Koutsaviti, Aikaterini

AU - Zhao, Yong

AU - Duan, Yaotao

AU - Harizani, Maria

AU - Miettinen, Karel

AU - Georgantea, Panagiota

AU - Rosenfeldt, Mads

AU - Viejo-Ledesma, Sara E.

AU - Petersen, Mikael Agerlin

AU - Bredie, Wender

AU - Stærk, Dan

AU - Roussis, Vassilios

AU - Ioannou, Efstathia

AU - Kampranis, Sotirios

PY - 2022

Y1 - 2022

N2 - Humankind relies on specialized metabolites for medicines, flavors, fragrances, and numerous other valuable biomaterials. However, the chemical space occupied by specialized metabolites, and, thus, their application potential, is limited because their biosynthesis is based on only a handful of building blocks. Engineering organisms to synthesize alternative building blocks will bypass this limitation and enable the sustainable production of molecules with non-canonical chemical structures, expanding the possible applications. Herein, we focus on isoprenoids and combine synthetic biology with protein engineering to construct yeast cells that synthesize 10 non-canonical isoprenoid building blocks with 16 carbon atoms. We identify suitable terpene synthases to convert these building blocks into C16 scaffolds and a cytochrome P450 to decorate the terpene scaffolds and produce different oxygenated compounds. Thus, we reconstruct the modular structure of terpene biosynthesis on 16-carbon backbones, synthesizing 28 different non-canonical terpenes, some of which have interesting odorant properties.

AB - Humankind relies on specialized metabolites for medicines, flavors, fragrances, and numerous other valuable biomaterials. However, the chemical space occupied by specialized metabolites, and, thus, their application potential, is limited because their biosynthesis is based on only a handful of building blocks. Engineering organisms to synthesize alternative building blocks will bypass this limitation and enable the sustainable production of molecules with non-canonical chemical structures, expanding the possible applications. Herein, we focus on isoprenoids and combine synthetic biology with protein engineering to construct yeast cells that synthesize 10 non-canonical isoprenoid building blocks with 16 carbon atoms. We identify suitable terpene synthases to convert these building blocks into C16 scaffolds and a cytochrome P450 to decorate the terpene scaffolds and produce different oxygenated compounds. Thus, we reconstruct the modular structure of terpene biosynthesis on 16-carbon backbones, synthesizing 28 different non-canonical terpenes, some of which have interesting odorant properties.

U2 - 10.1038/s41467-022-32921-w

DO - 10.1038/s41467-022-32921-w

M3 - Journal article

C2 - 36057727

VL - 13

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 5188

ER -

ID: 317358631