Fungal plasma membrane H+-ATPase inhibitory activity of o-hydroxybenzylated flavanones and chalcones from Uvaria chamae P. Beauv

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Fungal plasma membrane H+-ATPase inhibitory activity of o-hydroxybenzylated flavanones and chalcones from Uvaria chamae P. Beauv. / Kongstad, Kenneth Thermann; Wubshet, Sileshi Gizachew; Kjellerup, Lasse; winther, Anne-Marie Lund; Stærk, Dan.

I: Fitoterapia, Bind 105, 20.06.2015, s. 102-106.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Kongstad, KT, Wubshet, SG, Kjellerup, L, winther, A-ML & Stærk, D 2015, 'Fungal plasma membrane H+-ATPase inhibitory activity of o-hydroxybenzylated flavanones and chalcones from Uvaria chamae P. Beauv', Fitoterapia, bind 105, s. 102-106. https://doi.org/10.1016/j.fitote.2015.06.013

APA

Kongstad, K. T., Wubshet, S. G., Kjellerup, L., winther, A-M. L., & Stærk, D. (2015). Fungal plasma membrane H+-ATPase inhibitory activity of o-hydroxybenzylated flavanones and chalcones from Uvaria chamae P. Beauv. Fitoterapia, 105, 102-106. https://doi.org/10.1016/j.fitote.2015.06.013

Vancouver

Kongstad KT, Wubshet SG, Kjellerup L, winther A-ML, Stærk D. Fungal plasma membrane H+-ATPase inhibitory activity of o-hydroxybenzylated flavanones and chalcones from Uvaria chamae P. Beauv. Fitoterapia. 2015 jun. 20;105:102-106. https://doi.org/10.1016/j.fitote.2015.06.013

Author

Kongstad, Kenneth Thermann ; Wubshet, Sileshi Gizachew ; Kjellerup, Lasse ; winther, Anne-Marie Lund ; Stærk, Dan. / Fungal plasma membrane H+-ATPase inhibitory activity of o-hydroxybenzylated flavanones and chalcones from Uvaria chamae P. Beauv. I: Fitoterapia. 2015 ; Bind 105. s. 102-106.

Bibtex

@article{c9d316d42e5d42e69ea0f4f7374fba8e,
title = "Fungal plasma membrane H+-ATPase inhibitory activity of o-hydroxybenzylated flavanones and chalcones from Uvaria chamae P. Beauv",
abstract = "In our ongoing efforts of finding natural fungicides to fight food and feed spoilage during production and storage, the antifungal potential of Ghanaian Uvaria chamae P. Beauv. was investigated, with emphasis on plant metabolites targeting the fungal plasma membrane (PM) H+-ATPase. Ethyl acetate extract of U. chamae was subjected to high-resolution fungal PM H+-ATPase inhibition screening followed by structural elucidation by high-performance liquid chromatography–high-resolution mass spectrometry–solid-phase extraction–nuclear magnetic resonance spectroscopy (HPLC–HRMS–SPE–NMR). This led to identification of a series of uncommon o-hydroxybenzylated flavanones and chalcones, i.e., chamanetin (8), isochamanetin (9), isouvaretin (10), uvaretin (11), dichamanetin (12), and diuvaretin (15). Preparative-scale isolation of the active metabolites allowed determination of IC50 values for inhibition of the PM H+-ATPase, and growth inhibition of Saccharomyces cerevisiae and Candida albicans. These revealed a strong correlation between o-hydroxybenzyl substituents and PM H+-ATPase activity, with dichamanetin being the most potent compound, but showing moderate activity in the fungal growth inhibition assays.",
author = "Kongstad, {Kenneth Thermann} and Wubshet, {Sileshi Gizachew} and Lasse Kjellerup and winther, {Anne-Marie Lund} and Dan St{\ae}rk",
year = "2015",
month = jun,
day = "20",
doi = "10.1016/j.fitote.2015.06.013",
language = "English",
volume = "105",
pages = "102--106",
journal = "Fitoterapia",
issn = "1971-551X",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Fungal plasma membrane H+-ATPase inhibitory activity of o-hydroxybenzylated flavanones and chalcones from Uvaria chamae P. Beauv

AU - Kongstad, Kenneth Thermann

AU - Wubshet, Sileshi Gizachew

AU - Kjellerup, Lasse

AU - winther, Anne-Marie Lund

AU - Stærk, Dan

PY - 2015/6/20

Y1 - 2015/6/20

N2 - In our ongoing efforts of finding natural fungicides to fight food and feed spoilage during production and storage, the antifungal potential of Ghanaian Uvaria chamae P. Beauv. was investigated, with emphasis on plant metabolites targeting the fungal plasma membrane (PM) H+-ATPase. Ethyl acetate extract of U. chamae was subjected to high-resolution fungal PM H+-ATPase inhibition screening followed by structural elucidation by high-performance liquid chromatography–high-resolution mass spectrometry–solid-phase extraction–nuclear magnetic resonance spectroscopy (HPLC–HRMS–SPE–NMR). This led to identification of a series of uncommon o-hydroxybenzylated flavanones and chalcones, i.e., chamanetin (8), isochamanetin (9), isouvaretin (10), uvaretin (11), dichamanetin (12), and diuvaretin (15). Preparative-scale isolation of the active metabolites allowed determination of IC50 values for inhibition of the PM H+-ATPase, and growth inhibition of Saccharomyces cerevisiae and Candida albicans. These revealed a strong correlation between o-hydroxybenzyl substituents and PM H+-ATPase activity, with dichamanetin being the most potent compound, but showing moderate activity in the fungal growth inhibition assays.

AB - In our ongoing efforts of finding natural fungicides to fight food and feed spoilage during production and storage, the antifungal potential of Ghanaian Uvaria chamae P. Beauv. was investigated, with emphasis on plant metabolites targeting the fungal plasma membrane (PM) H+-ATPase. Ethyl acetate extract of U. chamae was subjected to high-resolution fungal PM H+-ATPase inhibition screening followed by structural elucidation by high-performance liquid chromatography–high-resolution mass spectrometry–solid-phase extraction–nuclear magnetic resonance spectroscopy (HPLC–HRMS–SPE–NMR). This led to identification of a series of uncommon o-hydroxybenzylated flavanones and chalcones, i.e., chamanetin (8), isochamanetin (9), isouvaretin (10), uvaretin (11), dichamanetin (12), and diuvaretin (15). Preparative-scale isolation of the active metabolites allowed determination of IC50 values for inhibition of the PM H+-ATPase, and growth inhibition of Saccharomyces cerevisiae and Candida albicans. These revealed a strong correlation between o-hydroxybenzyl substituents and PM H+-ATPase activity, with dichamanetin being the most potent compound, but showing moderate activity in the fungal growth inhibition assays.

U2 - 10.1016/j.fitote.2015.06.013

DO - 10.1016/j.fitote.2015.06.013

M3 - Journal article

C2 - 26102180

VL - 105

SP - 102

EP - 106

JO - Fitoterapia

JF - Fitoterapia

SN - 1971-551X

ER -

ID: 138960503