Possible artefacts in the in vitro determination of antimalarial activity of natural products that incorporate into lipid bilayer: apparent antiplasmodial activity of dehydroabietinol, a constituent of Hyptis suaveolens

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Standard

Possible artefacts in the in vitro determination of antimalarial activity of natural products that incorporate into lipid bilayer : apparent antiplasmodial activity of dehydroabietinol, a constituent of Hyptis suaveolens. / Ziegler, Hanne Lindvig; Jensen, Thomas Høgh; Christensen, Jette; Stærk, Dan; Hägerstrand, Henry; Sittie, Archibald A; Olsen, Carl Erik; Staalsø, Trine; Ekpe, Patrick; Jaroszewski, Jerzy W.

I: Planta Medica, Bind 68, 2002, s. 547-549.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Ziegler, HL, Jensen, TH, Christensen, J, Stærk, D, Hägerstrand, H, Sittie, AA, Olsen, CE, Staalsø, T, Ekpe, P & Jaroszewski, JW 2002, 'Possible artefacts in the in vitro determination of antimalarial activity of natural products that incorporate into lipid bilayer: apparent antiplasmodial activity of dehydroabietinol, a constituent of Hyptis suaveolens', Planta Medica, bind 68, s. 547-549. https://doi.org/10.1055/s-2002-32548

APA

Ziegler, H. L., Jensen, T. H., Christensen, J., Stærk, D., Hägerstrand, H., Sittie, A. A., Olsen, C. E., Staalsø, T., Ekpe, P., & Jaroszewski, J. W. (2002). Possible artefacts in the in vitro determination of antimalarial activity of natural products that incorporate into lipid bilayer: apparent antiplasmodial activity of dehydroabietinol, a constituent of Hyptis suaveolens. Planta Medica, 68, 547-549. https://doi.org/10.1055/s-2002-32548

Vancouver

Ziegler HL, Jensen TH, Christensen J, Stærk D, Hägerstrand H, Sittie AA o.a. Possible artefacts in the in vitro determination of antimalarial activity of natural products that incorporate into lipid bilayer: apparent antiplasmodial activity of dehydroabietinol, a constituent of Hyptis suaveolens. Planta Medica. 2002;68:547-549. https://doi.org/10.1055/s-2002-32548

Author

Ziegler, Hanne Lindvig ; Jensen, Thomas Høgh ; Christensen, Jette ; Stærk, Dan ; Hägerstrand, Henry ; Sittie, Archibald A ; Olsen, Carl Erik ; Staalsø, Trine ; Ekpe, Patrick ; Jaroszewski, Jerzy W. / Possible artefacts in the in vitro determination of antimalarial activity of natural products that incorporate into lipid bilayer : apparent antiplasmodial activity of dehydroabietinol, a constituent of Hyptis suaveolens. I: Planta Medica. 2002 ; Bind 68. s. 547-549.

Bibtex

@article{6f3466f00cea11df825d000ea68e967b,
title = "Possible artefacts in the in vitro determination of antimalarial activity of natural products that incorporate into lipid bilayer: apparent antiplasmodial activity of dehydroabietinol, a constituent of Hyptis suaveolens",
abstract = "Dehydroabietinol isolated from Hyptis suaveolens (L.) Poit. was found to inhibit growth of chloroquine-sensitive as well as chloroquine-resistant strains of Plasmodium falciparum cultivated in erythrocytes in vitro (IC 50 26-27 microM). However, erythrocytes exposed to dehydroabietinol were transformed in a dose-dependent manner towards spherostomatocytic forms with concomitant formation of endovesicles, as disclosed by transmission electron microscopy. The erythrocyte shape alterations caused by dehydroabietinol correlated well with its apparent IC 50 value. Thus, dehydroabietinol incorporates into the erythrocyte membrane, and since invasion and survival of Plasmodium parasites is known to depend on the function of the erythrocyte membrane, the observed antiplasmodial effect of dehydroabietinol is presumably an indirect effect on the host cell. Because of these findings, microscopic investigations should be generally used to support claims of antimalarial effects of apolar natural products.",
author = "Ziegler, {Hanne Lindvig} and Jensen, {Thomas H{\o}gh} and Jette Christensen and Dan St{\ae}rk and Henry H{\"a}gerstrand and Sittie, {Archibald A} and Olsen, {Carl Erik} and Trine Staals{\o} and Patrick Ekpe and Jaroszewski, {Jerzy W.}",
note = "Keywords: Animals; Antimalarials; Artifacts; Biological Products; Cell Size; Chloroquine; Diterpenes; Diterpenes, Abietane; Erythrocytes; Humans; Inhibitory Concentration 50; Lamiaceae; Lipid Bilayers; Microscopy, Electron; Plasmodium falciparum; Sesquiterpenes; Tumor Cells, Cultured",
year = "2002",
doi = "10.1055/s-2002-32548",
language = "English",
volume = "68",
pages = "547--549",
journal = "Planta Medica",
issn = "0032-0943",
publisher = "GeorgThieme Verlag",

}

RIS

TY - JOUR

T1 - Possible artefacts in the in vitro determination of antimalarial activity of natural products that incorporate into lipid bilayer

T2 - apparent antiplasmodial activity of dehydroabietinol, a constituent of Hyptis suaveolens

AU - Ziegler, Hanne Lindvig

AU - Jensen, Thomas Høgh

AU - Christensen, Jette

AU - Stærk, Dan

AU - Hägerstrand, Henry

AU - Sittie, Archibald A

AU - Olsen, Carl Erik

AU - Staalsø, Trine

AU - Ekpe, Patrick

AU - Jaroszewski, Jerzy W.

N1 - Keywords: Animals; Antimalarials; Artifacts; Biological Products; Cell Size; Chloroquine; Diterpenes; Diterpenes, Abietane; Erythrocytes; Humans; Inhibitory Concentration 50; Lamiaceae; Lipid Bilayers; Microscopy, Electron; Plasmodium falciparum; Sesquiterpenes; Tumor Cells, Cultured

PY - 2002

Y1 - 2002

N2 - Dehydroabietinol isolated from Hyptis suaveolens (L.) Poit. was found to inhibit growth of chloroquine-sensitive as well as chloroquine-resistant strains of Plasmodium falciparum cultivated in erythrocytes in vitro (IC 50 26-27 microM). However, erythrocytes exposed to dehydroabietinol were transformed in a dose-dependent manner towards spherostomatocytic forms with concomitant formation of endovesicles, as disclosed by transmission electron microscopy. The erythrocyte shape alterations caused by dehydroabietinol correlated well with its apparent IC 50 value. Thus, dehydroabietinol incorporates into the erythrocyte membrane, and since invasion and survival of Plasmodium parasites is known to depend on the function of the erythrocyte membrane, the observed antiplasmodial effect of dehydroabietinol is presumably an indirect effect on the host cell. Because of these findings, microscopic investigations should be generally used to support claims of antimalarial effects of apolar natural products.

AB - Dehydroabietinol isolated from Hyptis suaveolens (L.) Poit. was found to inhibit growth of chloroquine-sensitive as well as chloroquine-resistant strains of Plasmodium falciparum cultivated in erythrocytes in vitro (IC 50 26-27 microM). However, erythrocytes exposed to dehydroabietinol were transformed in a dose-dependent manner towards spherostomatocytic forms with concomitant formation of endovesicles, as disclosed by transmission electron microscopy. The erythrocyte shape alterations caused by dehydroabietinol correlated well with its apparent IC 50 value. Thus, dehydroabietinol incorporates into the erythrocyte membrane, and since invasion and survival of Plasmodium parasites is known to depend on the function of the erythrocyte membrane, the observed antiplasmodial effect of dehydroabietinol is presumably an indirect effect on the host cell. Because of these findings, microscopic investigations should be generally used to support claims of antimalarial effects of apolar natural products.

U2 - 10.1055/s-2002-32548

DO - 10.1055/s-2002-32548

M3 - Journal article

C2 - 12094303

VL - 68

SP - 547

EP - 549

JO - Planta Medica

JF - Planta Medica

SN - 0032-0943

ER -

ID: 17274513